Breast Cancer Kit

BRCAaccuTestTM

Total solution for genetic breast cancer BRCA genetic test(CE-IVD)

Introduction

NGS based rapid and reliable BRCA mutation detection panel & easy with convenient analysis software

BRCAaccuTestTM uses the next generation sequencing (NGS) to detect and analyze mutations in BRCA1 and BRCA2 genes. This product consists of reagents and analysis software to detect gene mutations.

With NGS, it is possible to detect various base sequence mutations quickly and stably, and with the sequence analysis software, anyone can easily obtain the analysis results at the clinical grade level.

Features
  • Detection of mutations in the whole exon region coding for BRCA1 and BRCA2 gene proteins
  • Experiment and decryption completed in 2 days
  • Using DNA extracted from blood
  • PCR amplicon-based library production
  • Provide data QC, analysis and automated variant information by using optimized analysis software for BRCA1 and 2 panels
  • Production and management from GMP and ISO 13485 certified organizations
  • IVD(CE) / RUO
Procedure
BRCAaccuTest04@2x-1-736x195
BRCA1 & 2 DNA Library Preparation
BRCAaccuTest05@2x
Specification
Category Contents
Target Design Target Region BRCA1 & BRCA2 protein coding exon region (about 17kb)
Amplicon Size 152 ~ 284 bp (average 230 bp)
Primer Pools 131 primer pairs
Sequencing Input DNA 10 ~ 50 ng
Samples 23 samples + 1 control DNA
Sequencer MiSeqDx, MiSeq
Reagent Kit MiSeq Reagent Nano Kit v2 300 cycles
Read Cycles 2 x 150 bp (paired-end reads)
Turn Around Time Total in 2 days : library prep (1 day) + sequencing run (1 day)
Sequencing Data Amplicon Coverage . Average coverage ≥ 200X . Minimum coverage ≥ 20X
Uniformity Amplicon over average coverage * 0.2 ≥ 98%
Variants Differentiate 100% of SNV, INDEL Heterozygous & homozygous mutations 
Analysis Solution NGeneAnalySys™ analysis software
Analyzing Time 1hr / sample
Information
Cat.No Contents Reactions Price

NGB111U

BRCAaccuTestTM

  • BRCA Amplicon Primer Mix 1
  • BRCA Amplicon Primer Mix 2
  • Hot Start PCR I Master Mix
  • Ligation Mix
  • Amplicon DNA Adapter
  • PCR II Barcoded Primer Mix N1~N24
  • PCR II Master Mix
  • Nuclease-Free Water
  • Control DNA, 5 ng/ul
  • Resuspension Buffer
  • Cleanup Beads
24

 

[Intended Use]

BRCAaccuTest™ is provided for research use only (RUO).

Not for use in diagnostic procedures.

Europe

BRCAaccuTest™ is provided for research (RUO) and diagnostic (CE-IVD) purposes.