Lung Cancer Kit

LUNGaccuTestTM

The simplest and most complete way to find gene mutations in lung cancer!

Introduction

LUNGaccuTest uses the next generation sequencing (NGS) to detect and analyze gene mutations that cause lung cancer. It detects a wide variety of mutations in 17 genes in a single experiment, dramatically simplifying existing complex lung cancer tests. LUNGaccuTest consists of reagent and gene mutation analysis software to detect mutations in lung cancer tissues.

Features
  • Detect SNV, INDEL, CNV, translocation mutations in one panel at a time,
  • Detection of 99% of mutations in lung cancer identified to date,
  • 65% non-small cell lung cancer patients, 80% lung adenocarcinoma patients can find the cause mutation
  • Provides analytical software results that can be simply and easily analyzed without NGS data analysis expert
Gene list of LUNGaccuTest

 Include some intron regions

EGFR KRAS BRAS ERBB2 MET NRAS ALK RET NRAS
AKT1 HRAS PIK3CA NTRK1 FGFR1 FGFR2 FGFR3 MAP2K1  
  • All exon of the gene + part of intron
  • Types of mutable detectable
  • Point mutation : EGFR, KRAS, NRAS, HRAS, BRAF, AKT1, PIK3CA, MAP2K1
  • Amplification: EGFR, KRAS, ERBB2, MET, PIK3CA, FGFR1
  • INDEL: EGFR, ERBB2, MET
  • Translocation: EGFR, ALK, RET, ROS1, NTRK1, FGFR1/2/3
Procedure
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LungAccu Test DNA Library Preparation
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Specification
Category Contents
Target Design Target Region 17 genes (all coding exons + 28 introns) 57 SNPs (sample ID)
Target size 0.1M bp
Probe type RNA
Probe size About 120mer
Sampleasdf Cancer Type Lung cancer
Sample type FFPE tissue, FF tissue
Tumor % ≥ 20%
Input DNA Quality DIN ≥ 3.0
Input DNA Quantity ≥ 200ng
Sequencing Target capture method  Hybridization (~120mer probes)
 Sequencer MiSeqDx, MiSeq
Reagent kit MiSeq® Reagent Kit v3(600 cycles)
Read Cycles 2 x 150 bp (paired-end reads)
Turn Around Time Total 5 days : library prep (3 days) + sequencing (2 days)
Reactions / Kit 20 samples + 1 control DNA
Sequencing Data  Data throughput ≥ 300M bp
Target coverage Average coverage ≥ 300X . Minimum coverage ≥ 100X
Variants SNV, INDEL, CNV, Translocation
Analysis Solution NGeneAnalySys™ analysis software
Analysis Time 2hrs / sample
Information
Cat.No Contents Reactions Price
NGB121U LUNGaccuTestTM 21