NGS(Next-generation sequencing) arrow

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Introduce

Genetic variants screening for hematologic malignancies

Acute Myeloid Leukemia (AML)

Acute Lymphoid Leukemia (ALL) Myelodysplastic/Myeloproliferative Neoplasm (MDS/MPN)

Multiple Myeloma (MM)

Lymphoma

Working Process

Specification

HEMEaccuTest™ DNA (CE-IVD) HEMEaccuTest™ RNA
Technique NGS (Next generation Sequencing)
Compatible platforms Illumina / Miseq, MiSeqDx
Target enrichment Targeted sequencing / Hyb & capture method
Specimen Blood, bone marrow and lymph nodes
Quantity 8 runs (8-12 samples, up to 96 samples/pack)
Analysis solution NgeneAnalySys™
Target genes 108 genes 53 genes (5 house-keeping genes)
Target size 305 kb 158 kb
Turn around time 3 days 3 ~ 3/5 days
Variants type SNV, INDEL (some SV) Fusion, Alternative splicing

Performance

Comparison of variants detection

13 clinical samples + 2 cell lines = 35 variants detected previously by Sanger sequencing, FISH, Real-time PCR, etc.

Concordance rate ≥ 99/9%

HEMEaccuTest™ DNA
MD MND Total
Conventional methods MD 35 0 35
MND 0 0 0
Total 35 0 35

MD : mutation detection / MND : mutation non-detection

Information

Cat no. Products Quantity Storage

NGB121UWW

NGB121UWW-71
NGB121UWW-73
NGB121UWW-72
NGB121UWW-74

HEMEaccuTest™ DNA (Approved CE-IVD)

  • DNA NGB Core-Library Prep Kit
  • NGB Core-Library Prep Index 1 Kit
  • NGB Core-Target Enrichment Kit
  • Target Enrichment Probe Kit
 96rxn
(8runs)		 -20 ℃

NGB122UWW

NGB122UWW-71
NGB122UWW-73
NGB122UWW-72
NGB122UWW-74

HEMEaccuTest™ RNA

  • RNA NGB Core-Library Prep Kit
  • NGB Core-Library Prep Index 1 Kit
  • NGB Core-Target Enrichment Kit
  • Target Enrichment Probe Kit
 96rxn
(8runs)		 -20 ℃
NGB312UWW NGeneAnalySys™ 1.4 (Software for both germline and somatic analysis)