Human Leukocyte Antigen Typing Kit

HLAaccuTest™ is an in vitro diagnostic medical device for HLA (human leukocyte antigen) typing that can identify high-resolution histocompatibility antigens using next-generation sequencing.

Organ transplant including hematopoietic stem cell transplant

Autoimmune disease including ankylosing spondylitis

Screening for chronic infectious diseases and a drug hypersensitivity reaction

Working Process

Specification

HLAaccuTest™
Specimen gDNA from blood or bone marrow aspirate
Input DNA 50~100 ng/locus
Target Enrichment method Amplicon (Long-range PCR)
Duration time of DNA library prep ~ 7.5hr
Target HLA locus Class I: A, B, C
Class II: DRB1/3/4/5, DQA1, DQB1, DPA1 and DPB1
Target region Entire Exon or specific Exon
Target size ~ 60 Kb
Ambiguity rate <0.2%
Sequencing Platform Illumina MiSeq / MiSeqDx
Sequencing Reagent MiSeq Reagent nano Kit, v2 ( up to 6 samples/run)
MiSeq Reagent micro Kit, v2 ( up to 24 samples/run)
Analysis software EasyHLAanalyzer™

Ordering information

Products Cat.no Quantity
RUO CE-IVD
HLAaccuTest™
& EasyHLAanalySys™

NGB151R-024

NGB151V-024

24 tests