Introduction
Human Leukocyte Antigen Typing Kit
HLAaccuTest™ All is an in vitro diagnostic medical device for HLA (human leukocyte antigen) typing that can identify high-resolution histocompatibility antigens using next-generation sequencing.
Organ transplant including hematopoietic stem cell transplant
Autoimmune disease including ankylosing spondylitis
Screening for chronic infectious diseases and a drug hypersensitivity reaction
Working Process
Specification
| HLAaccuTest™ All | |
|---|---|
| Specimen | gDNA from blood or bone marrow aspirate |
| Input DNA | 50~100 ng/locus |
| Target Enrichment method | Amplicon (Long-range PCR) |
| Duration time of DNA library prep | ~ 7.5hr |
| Target HLA locus | Class I: A, B, C Class II: DRB1/3/4/5, DQA1, DQB1, DPA1 and DPB1 |
| Target region | Entire Exon or specific Exon |
| Target size | ~ 60 Kb |
| Ambiguity rate | ＜0.2% |
| Sequencing Platform | Illumina MiSeq / MiSeqDx |
| Sequencing Reagent | MiSeq Reagent nano Kit, v2 ( up to 6 samples/run) MiSeq Reagent micro Kit, v2 ( up to 24 samples/run) |
| Analysis software | EasyHLAanalyzer™ |
Ordering information
| Products | Cat.no | Quantity | Storage | |
|---|---|---|---|---|
| RUO | CE-IVD (planned 2Q/2021) |
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|
HLAaccuTest™ All
|
NGB151R-024 |
NGB151V-024 |
24 tests |
-20℃ |
NGB151R-096 |
NGB151V-096 |
96 tests |
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