NGenePlex™ nCoV qRT-PCR Kit
NGenePlex™ nCoV qRT-PCR Kit is a reagent of real-time reverse transcription-polymerase chain reaction (RT-PCR) assay intended for the qualitatively detecting nucleic acid of SARS-CoV-2 from patients infected or suspected of COVID-19.
COVID-19 Test Workflow
NGenePlex™ nCoV qRT-PCR Kit is intended for use by qualified laboratory personnel specifically trained and instructed in qRT-PCR techniques as an in vitro diagnostic kit.
Also, intended use for detecting nucleic acid of SARS-CoV-2 from patients infected or suspected of COVID-19 with symptoms including fever, cough, fatigue, shortness of breath, and loss of smell and taste
Specimens can be collected from a respiratory tract such as a nasal, nasopharyngeal (NP), or oropharyngeal (OP) swab.
|NGenePlex™ nCoV qRT-PCR Kit|
|Target genes||RdRp and N genes on SARS-CoV-2|
|Specimen types||human nasopharyngeal (NP) swab, oropharyngeal (OP) swab|
|Validated Instruments*||CFX96™ Real-Time PCR Detection System (Bio-Rad)
ABI 7500 Real-Time PCR System (ThermoFisher Scientific)
QuantStudio 3 Real-Time PCR Instrument (ThermoFisher Scientific)
LightCycler® 480 System (Roche)
|Fluorometer channel||FAM (RdRp)
Cal Red 610/Texas Red (IC: human RNase P)
The primers and probes of NGenePlex™ nCoV qRT-PCR Kit were deigned to target the RdRP1 gene in the open reading frame (orf1ab) encoding non-structural proteins (nsp) for replication and the N gene encoding nucleocapsid protein. Also, an additional primer-probe set is included in the kit to detect the human RNase P gene as an internal control (IC).
This kit contains a one-step, ready-to-use system for the detection of 2019-nCoV by Reverse transcription Polymerase Chain Reaction (RT-PCR) in the real-time PCR system. The reagent mix contains a target sequences and target probes for the specific amplification of virus RNA. The reaction is done in one step real time RT-PCR, including followings;
A reverse transcription (RT), where the virus RNA is transcribed into cDNA.
Amplification (PCR), where a thermostable DNA polymerase is used to amplify the specific gene fragments.
Quantification, where fluorescence is emitted and measured by the real time systems´ optical unit during PCR.
The detection of amplified virus cDNA fragment is performed in fluorimeter channel FAM, channel HEX/VIC, and channel Cal Red 610/Texas Red/ROX with the fluorescent quencher NFQ-MGB.
|No.||Product Composition||Description||Cap or
|2X One-Step Reaction Mix||One-step real-time RT-PCR buffer||Clear||-20℃|
|One-Step Enzyme Mix||DNA polymerase, revers transcriptase||Red|
|Probe Mix||Primers and probes mix||Amber|
|Positive Control||Template for RdRp + N (SARS-nCoV-2) and RNase P (human)||Yellow|
|NGenePlex™ nCoV qRT-PCR Kit||96||-20℃|
|MVPrep™ Viral RNA/DNA Kit plus||100||RT|